Insertion junction: LMJ.RY0402.233484_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.233484
Chromosome:chromosome 17
Location:5646078
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g738250 (1 of 1) 2.5.1.31 - Ditrans,polycis-undecaprenyl-diphosphate synthase ((2E,6E)-farnesyl- diphosphate specific) / UPP synthetase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCAACAGTGCAGAGCAACGAACAGGCAGA
Internal bar code:TCGGGCATTGGGGGGCATGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:575
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGTTTGGCTATGCAGACA
Suggested primer 2:TTAGGTGAATTGGCGAAACC