| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.233490 |
| Chromosome: | chromosome 11 |
| Location: | 800541 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467639 | (1 of 1) PF04910 - Transcriptional repressor TCF25 (Tcf25) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATTGCTGTCGCAATGCAGGAGGACGAGG |
| Internal bar code: | CCGCGCTTACTAGTAAATTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 462 |
| LEAP-Seq percent confirming: | 99.9019 |
| LEAP-Seq n confirming: | 1018 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTTGACATCCACACACAG |
| Suggested primer 2: | ACGAGATCCTCCACCTCCTT |