| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.233492 |
| Chromosome: | chromosome 1 |
| Location: | 4885491 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g033900 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACTCCTTGACAATTGGGTGTGCCAACAG |
| Internal bar code: | GAGGGGGCGAACTGTGTGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 514 |
| LEAP-Seq percent confirming: | 99.3982 |
| LEAP-Seq n confirming: | 991 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGCTGATTACGCTTAGGG |
| Suggested primer 2: | TCCCTCTGTGTCAAGCCTCT |