Insertion junction: LMJ.RY0402.233493_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.233493
Chromosome:chromosome 6
Location:7941579
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g303483 (1 of 5) IPR001005//IPR009057//IPR017877 - SANT/Myb domain // Homeodomain-like // Myb-like domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTCCTCACTGTTCAATCCCATGACGCAGG
Internal bar code:TAATCTTGTCCTCGACTCAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:480
LEAP-Seq percent confirming:96.5644
LEAP-Seq n confirming:787
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CTCAGCCCAAAGGTTCTCAG
Suggested primer 2:TCATTAGCTTCCCGAGCACT