| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.233497 |
| Chromosome: | chromosome 13 |
| Location: | 2451740 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g579850 | (1 of 1) PTHR10984//PTHR10984:SF25 - ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN // ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN 3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGAATCGTCCTCGCGCTGTCCTCGTTTA |
| Internal bar code: | GTACAAAGTATGTGTTCTTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 606 |
| LEAP-Seq percent confirming: | 99.8089 |
| LEAP-Seq n confirming: | 5223 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGTCCCTTAGGAGGAGG |
| Suggested primer 2: | CACCCATCTGACGTGTTTTG |