| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.233501 |
| Chromosome: | chromosome 1 |
| Location: | 3498733 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g022350 | HEL3 | DEAH box ATP-dependent RNA helicase; (1 of 1) K14805 - ATP-dependent RNA helicase DDX24/MAK5 [EC:3.6.4.13] (DDX24, MAK5) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAAACCCAATGCTGGGCTGGGGAGATGG |
| Internal bar code: | GGGGTGTTCGAGTTGTGTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 236 |
| LEAP-Seq percent confirming: | 98.709 |
| LEAP-Seq n confirming: | 994 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTACTTCACGGGAGGGTT |
| Suggested primer 2: | CGTTGTTCTTCCTTGTGGGT |