| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.233516 |
| Chromosome: | chromosome 16 |
| Location: | 5637653 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679500 | NUOB8 | (1 of 1) K03946 - NADH dehydrogenase (ubiquinone) 1 alpha subcomplex subunit 2 (NDUFA2); NADH:ubiquinone oxidoreductase 11 kDa subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCAAAACGACGGCACGATGCGTTGTGCT |
| Internal bar code: | GGGGAGGTATCCGAGTAAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 621 |
| LEAP-Seq percent confirming: | 99.1259 |
| LEAP-Seq n confirming: | 1134 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAATTGATCTCGTTGCGCTG |
| Suggested primer 2: | GGTCCTTCAGCCCTCTCTCT |