Insertion junction: LMJ.RY0402.233522_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.233522
Chromosome:scaffold_24
Location:122104
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre24.g755997 PHC18,FAP150 Cell wall protein pherophorin-C18 antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):AAAGGTTGTTGCCGCGACACTGGCGCTTAC
Internal bar code:TTCGACTAGCGACGCACGCGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:648
LEAP-Seq percent confirming:99.2819
LEAP-Seq n confirming:553
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:AAAACTTCAATTTGGCGGTG
Suggested primer 2:CACCCTAATCCACATCCACC