| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.233552 |
| Chromosome: | chromosome 3 |
| Location: | 4487305 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176150 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAATTGCGGCTTTTGCGACTCCAATCAC |
| Internal bar code: | GCGTCGCGTAGGACGAGGTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 54 |
| LEAP-Seq percent confirming: | 99.4681 |
| LEAP-Seq n confirming: | 187 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTTCGCTTAAGAGGAGAA |
| Suggested primer 2: | TCTATCAGTACGTGCAGCCG |