Insertion junction: LMJ.RY0402.233556_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.233556
Chromosome:chromosome 9
Location:7615636
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g414800 CPY Serine carboxypeptidase; (1 of 1) K16297 - serine carboxypeptidase-like clade II [EC:3.4.16.-] (SCPL-II) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTGCCTTAGCAAGGCCACGTGCCCATGC
Internal bar code:AACCTATCATGCCGCCGGACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:651
LEAP-Seq percent confirming:99.2453
LEAP-Seq n confirming:1315
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:CTGATAGTGTGCATGACCGC
Suggested primer 2:CTTCTGATGCATGTCACGCT