| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.233562 |
| Chromosome: | chromosome 6 |
| Location: | 3863056 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278176 | (1 of 1) PTHR22847//PTHR22847:SF500 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTCACTGCCGCTTCCGTCGTTGTGTTG |
| Internal bar code: | GAATACGCTCAGGGGATGCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 412 |
| LEAP-Seq percent confirming: | 98.3169 |
| LEAP-Seq n confirming: | 2687 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGACACACTGTCGCACAA |
| Suggested primer 2: | AGGAAACAGCGAAGGTGCTA |