| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.233562 |
| Chromosome: | chromosome_9 |
| Location: | 7539138 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g414300 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCCAATCCGTTCAGGAACAAGACCCGCGTG |
| Internal bar code: | GCACGCAGGGCTCCCATCAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 435 |
| LEAP-Seq percent confirming: | 99.2509 |
| LEAP-Seq n confirming: | 1060 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGTCCTCTTGCACGTAT |
| Suggested primer 2: | AGAGGTTGCGCCTCAAAGTA |