Insertion junction: LMJ.RY0402.233572_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.233572
Chromosome:chromosome 5
Location:3414896
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g241202 (1 of 3) 3.4.22.53 - Calpain-2 / Milli-calpain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGATTACTCCCTTCTCCATGAACGGCTATC
Internal bar code:TATGAACTGGATTGTAGTTTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1048
LEAP-Seq percent confirming:99.4331
LEAP-Seq n confirming:7718
LEAP-Seq n nonconfirming:44
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:ACGTCAATCATTTGTTGCGA
Suggested primer 2:ATGGGCGATGTAAGCAAGTC