| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.233572 |
| Chromosome: | chromosome 5 |
| Location: | 3414915 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241202 | (1 of 3) 3.4.22.53 - Calpain-2 / Milli-calpain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGCGGAGGAGTAGCACGACGTGTGTGGG |
| Internal bar code: | TCCAAGGCGAGGTACCTGTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 116 |
| LEAP-Seq percent confirming: | 99.6644 |
| LEAP-Seq n confirming: | 297 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTCAATCATTTGTTGCGA |
| Suggested primer 2: | ATGGGCGATGTAAGCAAGTC |