| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.233590 |
| Chromosome: | chromosome 14 |
| Location: | 3964410 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g633250 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGACGCATTGTGCCAGCACTTGTGAACGG |
| Internal bar code: | CTTGGGCGCGTCAAGTATATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 216 |
| LEAP-Seq percent confirming: | 59.8162 |
| LEAP-Seq n confirming: | 716 |
| LEAP-Seq n nonconfirming: | 481 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCTTCCTCAACACAACGG |
| Suggested primer 2: | GCACGGACCCCTGAGTAATA |