Insertion junction: LMJ.RY0402.233631_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.233631
Chromosome:chromosome 16
Location:2063166
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g657250 GOX17,GOX19 Glyoxal oxidase 19; (1 of 12) 1.1.3.9 - Galactose oxidase / Beta-galactose oxidase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCCAACCGTGTTTGACCACCTAGAAGAG
Internal bar code:GGCCGACCCGGTGATCGCGTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:653
LEAP-Seq percent confirming:99.4519
LEAP-Seq n confirming:3266
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:TGTAGATGAAGAGCATGGCG
Suggested primer 2:CGCATCAGCTCTCCATAACA