Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.233631 |
Chromosome: | chromosome 16 |
Location: | 2063166 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g657250 | GOX17,GOX19 | Glyoxal oxidase 19; (1 of 12) 1.1.3.9 - Galactose oxidase / Beta-galactose oxidase | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCAACCGTGTTTGACCACCTAGAAGAG |
Internal bar code: | GGCCGACCCGGTGATCGCGTAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 653 |
LEAP-Seq percent confirming: | 99.4519 |
LEAP-Seq n confirming: | 3266 |
LEAP-Seq n nonconfirming: | 18 |
LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTAGATGAAGAGCATGGCG |
Suggested primer 2: | CGCATCAGCTCTCCATAACA |