| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.233640 |
| Chromosome: | chromosome 13 |
| Location: | 4216058 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g592550 | (1 of 2) PF13661 - 2OG-Fe(II) oxygenase superfamily (2OG-FeII_Oxy_4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGTGGGTGGTGGTGGTGATGGTGGTGG |
| Internal bar code: | GCGCCAAGCAGAGAGGACGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 608 |
| LEAP-Seq percent confirming: | 90.8523 |
| LEAP-Seq n confirming: | 2036 |
| LEAP-Seq n nonconfirming: | 205 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACTTTTTCTCCGAGGCTG |
| Suggested primer 2: | CTTGCTTCCTCTCGTCATCC |