| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.233653 |
| Chromosome: | chromosome 3 |
| Location: | 5927404 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g190150 | CGL1 | (1 of 2) PF11833 - Protein of unknown function (DUF3353) (DUF3353); Conserved in the Green Lineage | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAATGTTGCCGCGGGAACGCAAAGGCATGA |
| Internal bar code: | AGTCTGCCGGCCGATCCTGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 450 |
| LEAP-Seq percent confirming: | 99.2453 |
| LEAP-Seq n confirming: | 526 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTGCCAACCAGTATTCCT |
| Suggested primer 2: | ACTGCGATGCATACAAGCAC |