| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.233653 |
| Chromosome: | chromosome 13 |
| Location: | 3371782 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g586800 | (1 of 8) IPR001810//IPR006553 - F-box domain // Leucine-rich repeat, cysteine-containing subtype | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGACCCTACCTGGCGGGCCAAAGGCAT |
| Internal bar code: | CAGGACCTTACGACATGGATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 493 |
| LEAP-Seq percent confirming: | 98.9486 |
| LEAP-Seq n confirming: | 4988 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATAGGAGGAAACCAACGC |
| Suggested primer 2: | CTGTACTGGCGTGCTGATGT |