| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.233658 |
| Chromosome: | chromosome 2 |
| Location: | 4426825 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g099850 | PDC2 | (1 of 1) PTHR11516:SF25 - PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA-3, CHLOROPLASTIC; Pyruvate dehydrogenase, E1 component, alpha subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTAGTTAAGGAAGTGGTGGGGGAAGGGGG |
| Internal bar code: | GCCAAAACGGATGTTCACTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 70 |
| LEAP-Seq percent confirming: | 96.4286 |
| LEAP-Seq n confirming: | 108 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCCAAGATCGCAGCTAAC |
| Suggested primer 2: | ATCCCGCAGCTGAAGAAGTA |