| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.233659 |
| Chromosome: | chromosome 16 |
| Location: | 2223685 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g658750 | (1 of 4) PTHR14255//PTHR14255:SF3 - ATP-DEPENDENT PROTEASE CEREBLON // SULFITE EXPORTER TAUE/SAFE FAMILY PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATTCCTCCCTCTGTCGAGCCCAGACTTC |
| Internal bar code: | GAAACTCCGCTCGGGGCAGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1200 |
| LEAP-Seq percent confirming: | 98.7113 |
| LEAP-Seq n confirming: | 383 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGAGCCTGAGTGTGGTTC |
| Suggested primer 2: | CCATAGTGTGCGAGCCTGTA |