Insertion junction: LMJ.RY0402.233662_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.233662
Chromosome:chromosome 12
Location:5157748
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g527250 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTAAATGCGCGCATGCGCGGGGAGAGGGT
Internal bar code:ACGGGTGATCGTGTGCTCGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1024
LEAP-Seq percent confirming:99.4305
LEAP-Seq n confirming:8729
LEAP-Seq n nonconfirming:50
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:AACAGTGCACACCAACCAAA
Suggested primer 2:GCACGGTAGGTTGACACCTT