| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.233674 |
| Chromosome: | chromosome 12 |
| Location: | 5643485 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532300 | APG10,ATG10 | Autophagy-related protein; (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAAGACAACGAGCACGAGAAGGAGGGCA |
| Internal bar code: | GGCCAAAGATGACCGCAAAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 765 |
| LEAP-Seq percent confirming: | 96.2004 |
| LEAP-Seq n confirming: | 5798 |
| LEAP-Seq n nonconfirming: | 229 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGTGTTCATGGCTTGGAA |
| Suggested primer 2: | CACAGCGGTGGTAGTGTTTG |