Insertion junction: LMJ.RY0402.233691_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.233691
Chromosome:chromosome 1
Location:4654557
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g032000 (1 of 19) IPR000225//IPR016024 - Armadillo // Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGAGGTGCGGAGTGCGGAATGATGAGCTA
Internal bar code:TTGTCGGCCAAGCTTGGATCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:365
LEAP-Seq percent confirming:99.4652
LEAP-Seq n confirming:186
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGAACCAACCCAACCAAC
Suggested primer 2:CCTTGCGTCAAGTAGGAAGC