| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.233721 |
| Chromosome: | chromosome 1 |
| Location: | 2588535 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g015050 | AGK1 | (1 of 1) IPR006569//IPR017956//IPR020478 - CID domain // AT hook, DNA-binding motif // AT hook-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCATTTCCGTACCACCCCACGCGCGCGTG |
| Internal bar code: | CTGGATTCGGGCGTCGCGAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 99.6949 |
| LEAP-Seq n confirming: | 2614 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACTGCCGTCTGTGTTGT |
| Suggested primer 2: | GGTGTCTGGTTTGAGGTCGT |