Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.233737 |
Chromosome: | chromosome_17 |
Location: | 3391745 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g723950 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCTTACCGCGGCTCCTCGTTGTTCTCCAGG |
Internal bar code: | GAGCGGCTGGCTTTGTGGGTTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 649 |
LEAP-Seq percent confirming: | 99.4898 |
LEAP-Seq n confirming: | 780 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTGGCTCCAACCTAGCAGTC |
Suggested primer 2: | CCAAAACATCATGACAACGC |