| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.233737 |
| Chromosome: | chromosome 17 |
| Location: | 3391745 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723950 | FAP397 | Flagellar Associated Protein 397 with UDP-galactose 4-epimerase domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTACCGCGGCTCCTCGTTGTTCTCCAGG |
| Internal bar code: | GAGCGGCTGGCTTTGTGGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 649 |
| LEAP-Seq percent confirming: | 99.4898 |
| LEAP-Seq n confirming: | 780 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGCTCCAACCTAGCAGTC |
| Suggested primer 2: | CCAAAACATCATGACAACGC |