| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.233749 |
| Chromosome: | chromosome 6 |
| Location: | 8029303 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304150 | (1 of 4) PF06985//PF12796 - Heterokaryon incompatibility protein (HET) (HET) // Ankyrin repeats (3 copies) (Ank_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGACGGCCCCGCCTGCGACACGGGAGCAA |
| Internal bar code: | TCCCGGGCGACACCAGGGTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 567 |
| LEAP-Seq percent confirming: | 99.5122 |
| LEAP-Seq n confirming: | 816 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCAGCTACAGCTTCGGAT |
| Suggested primer 2: | CGCTGACTGTCTACACCGAA |