| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.233752 |
| Chromosome: | chromosome 6 |
| Location: | 5951497 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g288800 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCGGAGTCGACGCCTCTGCACACGGCAG |
| Internal bar code: | GAACGACTTACACTCTCGACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1227 |
| LEAP-Seq percent confirming: | 99.6564 |
| LEAP-Seq n confirming: | 580 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCACCTCTCAGCTTCAAAC |
| Suggested primer 2: | GTCGCTCTTTCCTCCTTCCT |