| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.233756 |
| Chromosome: | chromosome 2 |
| Location: | 1419163 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g083450 | (1 of 1) IPR000104//IPR002938 - Antifreeze protein, type I // FAD-binding domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCACCGCCATACGGTTGCATCACATGC |
| Internal bar code: | TCCGTGTGGGATTACCGGCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 644 |
| LEAP-Seq percent confirming: | 98.8201 |
| LEAP-Seq n confirming: | 6700 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 92 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTTTCAGATGCACCCTA |
| Suggested primer 2: | TGAGGTGAGATACGTGAGCG |