| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.233760 |
| Chromosome: | chromosome 16 |
| Location: | 5444765 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681051 | (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCTTTGAGCCCATGGAGGAGCTGTTTTG |
| Internal bar code: | TCAAGTGACTTAACTGAATGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 361 |
| LEAP-Seq percent confirming: | 99.5055 |
| LEAP-Seq n confirming: | 3622 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAGCCTTTGTAGCGTTGC |
| Suggested primer 2: | ATCACTGTGTTGGTGCTGGA |