| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.233761 |
| Chromosome: | chromosome 8 |
| Location: | 4378012 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g381850 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGGGAGGGGGGGCGACGTTGATAGACAC |
| Internal bar code: | GGTAAATACAAGGCTGGCACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 570 |
| LEAP-Seq percent confirming: | 98.6129 |
| LEAP-Seq n confirming: | 782 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCCAGCTAGTTCAGTCGC |
| Suggested primer 2: | CATCACACCTCACAATTCGG |