| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.233774 |
| Chromosome: | chromosome_13 |
| Location: | 3357601 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g586750 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAGAAGTACTGCTAGACCGCCGCAACTGGA |
| Internal bar code: | ATGCTGGATCTGGCGGGCAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 590 |
| LEAP-Seq percent confirming: | 99.2218 |
| LEAP-Seq n confirming: | 2295 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGATGGAAACCGTGAGG |
| Suggested primer 2: | GGCAAAAGCATGCCTCTAAG |