Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.233774 |
Chromosome: | chromosome_13 |
Location: | 3357601 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g586750 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTCTCTCCCAACTTCCCCAGTGCAGTCGGC |
Internal bar code: | GTTACTCATAACAGTTGCGTCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1001 |
LEAP-Seq percent confirming: | 98.8915 |
LEAP-Seq n confirming: | 13293 |
LEAP-Seq n nonconfirming: | 149 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACTTGATGGAAACCGTGAGG |
Suggested primer 2: | GGCAAAAGCATGCCTCTAAG |