Insertion junction: LMJ.RY0402.233779_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.233779
Chromosome:chromosome 2
Location:5816327
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g111000 RRA1,CGL9 (1 of 3) PTHR10994//PTHR10994:SF3 - RETICULON // ARABINOSYLTRANSFERASE; Reduced residual arabinose 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGACAGTGCGCCAACCTCGGTCTCAGCGC
Internal bar code:GGGTTTCCCCGATCACCACGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:590
LEAP-Seq percent confirming:98.7219
LEAP-Seq n confirming:4171
LEAP-Seq n nonconfirming:54
LEAP-Seq n unique pos:64

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGAAGAAACAGAGCCAGC
Suggested primer 2:CTCAAAGGAGGCGTTCAGTC