| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.233785 |
| Chromosome: | chromosome 12 |
| Location: | 5840321 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533650 | (1 of 1) PTHR13439//PTHR13439:SF0 - CT120 PROTEIN // BCDNA.GH12326 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTATACCGTATTCAAGGCAGCATATGCT |
| Internal bar code: | ACGCAAAGAGGGACCGGTGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 509 |
| LEAP-Seq percent confirming: | 97.2973 |
| LEAP-Seq n confirming: | 864 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTATGAGCGAATGGTGGA |
| Suggested primer 2: | GTGTTGCCTTGCGAAGTACA |