| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.233788 |
| Chromosome: | chromosome 3 |
| Location: | 3063857 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g164450 | (1 of 100) IPR001810 - F-box domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGTGGGTGGTGTGGGTGCTGTGGGTGC |
| Internal bar code: | CGTGCGTCGGCTTACGAGCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 313 |
| LEAP-Seq percent confirming: | 95.5556 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCGCTGTTTCATCTTTGG |
| Suggested primer 2: | ACGGCCTGCTCAAACTAAGA |