| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.233797 |
| Chromosome: | chromosome 10 |
| Location: | 1259157 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426850 | (1 of 3) PF02458 - Transferase family (Transferase) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCAATCACCGATCCCTTCATCTCCCCA |
| Internal bar code: | GTGGCTGGGAACAGGAAGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 405 |
| LEAP-Seq percent confirming: | 99.499 |
| LEAP-Seq n confirming: | 2582 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACTTGAAGAGGCCTTGAC |
| Suggested primer 2: | CGAGAAGAGTGTCAGGAGGG |