Insertion junction: LMJ.RY0402.233809_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.233809
Chromosome:chromosome 8
Location:935794
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g361850 (1 of 1) PF05981 - CreA protein (CreA) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCCCACACGTCTTAGGCGAGGTGCCGTG
Internal bar code:CCTGGGTCGCCACCGAGATTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:770
LEAP-Seq percent confirming:98.8421
LEAP-Seq n confirming:2561
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:57

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTTCTTTGCTTGTGGCTC
Suggested primer 2:GGTGATAGGCGCAATCAAGT