| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.233842 |
| Chromosome: | chromosome 4 |
| Location: | 4075103 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g233202 | (1 of 14) PF04577 - Protein of unknown function (DUF563) (DUF563) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGGACGGGGGTGTTGTGGTCCGTGCAG |
| Internal bar code: | TGGGCACAGTCATCGCGCTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 774 |
| LEAP-Seq percent confirming: | 99.5596 |
| LEAP-Seq n confirming: | 9947 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAATGTACACGTGTGAGG |
| Suggested primer 2: | CTCGTACATGTGGTTGTGGC |