Insertion junction: LMJ.RY0402.233853_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.233853
Chromosome:chromosome 4
Location:2861238
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g224400 (1 of 1) 3.6.3.25//3.6.3.44 - Sulfate-transporting ATPase // Xenobiotic-transporting ATPase / Steroid-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGGGATGAAGGTTTGCCAGTTGGTTCAG
Internal bar code:ATTGTCTTATGTGACTACTACT

Confirmation - LEAP-Seq

LEAP-Seq distance:805
LEAP-Seq percent confirming:99.4862
LEAP-Seq n confirming:14134
LEAP-Seq n nonconfirming:73
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCGCATTTCTATTCCTGC
Suggested primer 2:GTGATCTCCAGCAGGAAGGA