| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.233858 |
| Chromosome: | chromosome 3 |
| Location: | 4162205 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173700 | KIL14 | Putative kinesin-like motor protein; (1 of 1) K10396 - kinesin family member 5 (KIF5) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGAGAAGCAGAAGGTGCACGGCCTGAAG |
| Internal bar code: | TCCATTGCTTCTCCCGGCGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 771 |
| LEAP-Seq percent confirming: | 98.2933 |
| LEAP-Seq n confirming: | 3686 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCAAACCGTTATCCAGTG |
| Suggested primer 2: | CACACACACACACACACCGT |