Insertion junction: LMJ.RY0402.233862_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.233862
Chromosome:chromosome 12
Location:8422491
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g548550 (1 of 1) PF05742 - Transport and Golgi organisation 2 (TANGO2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACGGGGGACGCGTCGCCCGCGAATTTTGC
Internal bar code:TGGCACCGTCTCGTGGAGTTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:561
LEAP-Seq percent confirming:82.3279
LEAP-Seq n confirming:1351
LEAP-Seq n nonconfirming:290
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:AGTGGTCACATGTGTTCGGA
Suggested primer 2:GAAGCAGGTTGCAGGAAGTC