| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.233868 |
| Chromosome: | chromosome 12 |
| Location: | 6360670 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537500 | (1 of 1) PTHR11200//PTHR11200:SF136 - INOSITOL 5-PHOSPHATASE // PHOSPHOINOSITIDE PHOSPHATASE SAC1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCTCACTGAGGGGATGCGGAGAAGCAGT |
| Internal bar code: | AACGTGCAACCGGTTCATATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 557 |
| LEAP-Seq percent confirming: | 99.4219 |
| LEAP-Seq n confirming: | 12898 |
| LEAP-Seq n nonconfirming: | 75 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGACAGTGAAGTAAGGGG |
| Suggested primer 2: | ACACACACACACACACCACG |