| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.233871 |
| Chromosome: | chromosome 13 |
| Location: | 2238427 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578600 | (1 of 1) PTHR35464//PTHR35464:SF1 - FAMILY NOT NAMED // F18O14.9 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCGGTGATGCTGTCGGCACACCACACCA |
| Internal bar code: | CTAACCGCTATATATTACGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 234 |
| LEAP-Seq percent confirming: | 98.41 |
| LEAP-Seq n confirming: | 11945 |
| LEAP-Seq n nonconfirming: | 193 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGAGGAAGGATTCGTGG |
| Suggested primer 2: | ACACCTCAGGCCCTTACCTT |