| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.233905 |
| Chromosome: | chromosome 2 |
| Location: | 929242 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g079700 | PYR2 | (1 of 1) 2.1.3.2 - Aspartate carbamoyltransferase / Carbamylaspartotranskinase; Aspartate carbamoyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGACCTACCGTACGTTACTGGGGGGCAT |
| Internal bar code: | AGTCAAGATGATATGGGTCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 635 |
| LEAP-Seq percent confirming: | 87.2467 |
| LEAP-Seq n confirming: | 1464 |
| LEAP-Seq n nonconfirming: | 214 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACAAATATTGTGTGGGGC |
| Suggested primer 2: | TTCAACACTGGTGGCATCAT |