Insertion junction: LMJ.RY0402.233906_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.233906
Chromosome:chromosome_16
Location:82507
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre16.g695700 antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GGAACGAAGCCAGGCATGCATGGTCATTGA
Internal bar code:CACACGTGCGCCACGTATCCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1087
LEAP-Seq percent confirming:96.3904
LEAP-Seq n confirming:721
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CTTAGCCTTGCCAGAGATGG
Suggested primer 2:GGAAACCGCAGTACCCTGTA