Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.233906 |
Chromosome: | chromosome_16 |
Location: | 82507 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g695700 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGAACGAAGCCAGGCATGCATGGTCATTGA |
Internal bar code: | CACACGTGCGCCACGTATCCCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1087 |
LEAP-Seq percent confirming: | 96.3904 |
LEAP-Seq n confirming: | 721 |
LEAP-Seq n nonconfirming: | 27 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTTAGCCTTGCCAGAGATGG |
Suggested primer 2: | GGAAACCGCAGTACCCTGTA |