| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.233908 |
| Chromosome: | chromosome 10 |
| Location: | 1121879 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425600 | PGM15 | Haloacid dehalogenase-like hydrolase (HAD) superfamily protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCACGCCTCCGCCCAGCTCTCCACACG |
| Internal bar code: | ACTTGGTAACTTGGATTTCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 106 |
| LEAP-Seq percent confirming: | 99.1759 |
| LEAP-Seq n confirming: | 21302 |
| LEAP-Seq n nonconfirming: | 177 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACGTGAAGCTGGATGAAG |
| Suggested primer 2: | TTATAGTGAGGCCAAACCCG |