| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.233929 |
| Chromosome: | chromosome 11 |
| Location: | 2533000 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g476026 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGAGGGAAGCCTAAGTCCTAGGAGAGCA |
| Internal bar code: | CCCATAAGTCTGCAGTCTCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 860 |
| LEAP-Seq percent confirming: | 98.778 |
| LEAP-Seq n confirming: | 7679 |
| LEAP-Seq n nonconfirming: | 95 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTCCCTGCTGTTTGGTTG |
| Suggested primer 2: | GCGAGCAAAATCATATCGGT |