| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.233938 |
| Chromosome: | chromosome 17 |
| Location: | 397407 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g698800 | (1 of 1) K06664 - peroxin-2 (PEX2, PXMP3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCATCTGCTGGGGTCGGGGAATCAGCTGC |
| Internal bar code: | GGTCGCCAAGTCGAGCCGCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 540 |
| LEAP-Seq percent confirming: | 53.2694 |
| LEAP-Seq n confirming: | 13165 |
| LEAP-Seq n nonconfirming: | 11549 |
| LEAP-Seq n unique pos: | 127 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTTGTGGAGTTGATGGG |
| Suggested primer 2: | CGCCACGTGTTCTGCTACTA |