| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.233947 |
| Chromosome: | chromosome 1 |
| Location: | 593619 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003463 | (1 of 1) K08735 - DNA mismatch repair protein MSH2 (MSH2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGGACAAAGGGAGGAAGGGGTAAGAGC |
| Internal bar code: | TGGGTTCGATCCTGTCAGCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 304 |
| LEAP-Seq percent confirming: | 98.2326 |
| LEAP-Seq n confirming: | 12061 |
| LEAP-Seq n nonconfirming: | 217 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAAGTGAAGGCGTAGCCA |
| Suggested primer 2: | GGGAGGGGGATTACATTCAT |